High-Performance Liquid Chromatography (HPLC)

What is HPLC?

High-Performance Liquid Chromatography (HPLC) is a separation and quantification technique used to analyze components in liquid samples. It identifies and measures target compounds by pushing a solution through a chromatographic column under high pressure.

HPLC is essential for analyzing polymers, pharmaceuticals, biomolecules, additives, impurities, and degradation products.

Use of HPLC

• Quantification of active compounds
• Detection of impurities and residual monomers
• Polymer degradation and additive analysis
• Drug release and stability studies
• Leachables and extractables analysis

Applications of HPLC

• Pharmaceuticals: potency, impurity profiling
• Polymers: monomer residuals, stabilizers, degradation products
• Biomaterials: hydrogel degradation, release studies
• Food & Chemicals: ingredient verification

Sample Analysis Process

1. Submission & Review

Identify analytes, solvents, and detection needs

2. Sample Preparation

Filtration, dilution, or extraction

3. HPLC Measurement

UV, fluorescence, or mass spectrometry detection
• Custom gradient and isocratic runs

4. Reporting

Chromatograms
• Quantification curves
• Impurity profiles

Why Choose Materials Metric for Your HPLC Analysis

• Accurate quantification and trace-level detection
• Specialized expertise in complex polymer and pharmaceutical samples
• Multiple detection modes (UV, fluorescence, MS)
• Support for extractables, leachables, and stability studies
• Clear reporting with quantitative results and interpretation

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